The team will gather in Miami beginning on Aug. 17 and will depart for Guatemala City the following day.
Four members of the Olympic team that was ousted in the first round in China are in Bradley's squad: his son, Michael Bradley, Sacha Kljestan, Maurice Edu and goalkeeper Brad Guzan.
Eleven players on the roster are European-based, including keeper Tim Howard, who missed the qualifiers against Barbados with a back injury.
The USA has a 10-4-5 win-loss-tie record against Guatemala and has shut out the Chapines in their last six games.
The USA is unbeaten against Guatemala in World Cup qualifying.
U.S. Roster vs. Guatemala
(2010 FIFA World Cup Qualifier, Aug. 20)
Brad Guzan (Aston Villa/ENG), Tim Howard (Everton/ENG)
Carlos Bocanegra (Rennes/FRA), Steve Cherundolo (Hannover 96/GER), Jay DeMerit (Watford/ENG), Frankie Hejduk (Columbus Crew), Oguchi Onyewu (Standard Liege/BEL), Heath Pearce (Hansa Rostock/GER).
Michael Bradley (Heerenveen/NED), Maurice Edu (Toronto FC), Sacha Kljestan (Chivas USA), Eddie Lewis (Derby County/ENG), Pablo Mastroeni (Colorado Rapids)
Brian Ching (Houston Dynamo), Landon Donovan (Los Angeles Galaxy), Clint Dempsey (Fulham/ENG), Eddie Johnson (Fulham/ENG).