Preki succeeded Bob Bradley in 2007 and was named MLS Coach of the Year that season. He posted a career record of 40-29-21 (W-L-T) and a winning percentage of .561 with Chivas USA.
"Preki has brought a great deal of success to this team, and is an important part of our history," said Chivas USA Vice President of Soccer Operations Stephen Hamilton. "His dedication and expertise have led us to the playoffs in each of his seasons here, and we owe him our thanks for all he has given the club. We wish him the very best as he moves into the next phase of his coaching career."
Preki had served as Bradley's assistant in 2006.
"It has been an honor to be a part of the Chivas USA family for the last four years," said Preki. "In my time here I have grown as a coach, and I am proud of our accomplishments. While I am excited to take the next step in my career, I will always have good memories of my time with Chivas USA."
Chivas USA's practices on Friday and Monday will be led by assistant coach Carlos Llamosa, who will be in charge until the club names a head coach.