The squad features two four-time honorees: Shalrie Joseph (New England) and Dwayne De Rosario (Toronto FC).
Landon Donovan (LA Galaxy) and Jeff Cunningham (FC Dallas) were named to the team for a third time.
Chivas USA goalkeeper Zach Thornton, the Goalkeeper of the Year and Comeback Player of the Year, earned his third major award of this year and his second career Best XI nod.
Columbus defender Chad Marshall, this year's Visa Defender of the Year, is a two-time Best XI member.
The Best XI members received the most votes from the media, MLS players and MLS coaches and general managers.