Captain Aaron Mokoena of English club Blackburn Rovers will lead the Bafana Bafana in the middle of the defense next to Nasief Morris of Greek club Panathinaikos.
Other EPL players include Everton's Steven Pienaar, who is playing on loan from Borussia Dortmund, and Mokoena's Blackburn teammate, Benni McCarthy.
German club Arminia Bielefeld leads all teams with three players represented on the Bafana Bafana: goalkeeper Rowen Fernandez, midfielder Siyabonga Nkosi and striker Sibusiso Zuma, who scored his first goal of the season in stoppage time of Sunday's 3-1 win over Nuremberg.
Other players have been recalled from Israel, Russia and Sweden.
South Africa hosts Canada next Tuesday in Durban.
South Africa Roster
GOALKEEPERS (2): Rowen Fernandez (Arminia Bielefeld/GER), Moeneeb Josephs (Wits University)
DEFENDERS (7): Bradley Carnell (Karlsruhe/GER), Tsepo Masilela (Maccabi Haifa/ISR), Vuyo Mere (Mamelodi Sundowns), Benson Mhlongo (Mamelodi Sundowns), Aaron Mokoena (Blackburn Rovers/ENG), Bryce Moon (Ajax Cape Town), Nasief Morris (Panathinaikos/GRE)
MIDFIELDERS (9): Delron Buckley (Borussia Dortmund/GER), Brent Carelse (Mamelodi Sundowns), Lance Davids (Djurgardens/SWE), Kagisho Dikgacoi (Lamontville Golden Arrows), Teko Modise (Orlando Pirates), Siyabonga Nkosi (Arminia Bielefeld/GER), Steven Pienaar (Everton/ENG), Macbeth Sibaya (Rubin Kazan/RUS), Siphiwe Tshabalala (Kaizer Chiefs)
FORWARDS (4): Lerato Chabangu (Mameodi Sundowns), Thembinkosi Fanteni (Ajax Cape Town), Benedict McCarthy (Blackburn Rovers/ENG), Sibusiso Zuma (Arminia Bielefeld/GER).