Dest became the 10th American to score in UEFA Champions League, racing through the Kiev defense to take the ball and score on an angled shot.
Barca coach Ronald Koeman rested Lionel Messi and Frenkie de Jong, who both stayed behind, and the move paid off as Barcelona clinched a berth in the round of 16 with two games to spare.
Dest earned his third start in this season's Champions League after right back Sergi Roberto was injured in Saturday's 1-0 loss to Atletico Madrid.
"Dest comes with a lot of desire," said Barcelona left back Jordi Alba. "With Sergi Roberto's unfortunate injury he will surely have more opportunities. I think he has a high level. He has a great future and fits perfectly with the characteristics that a Barcelona fullback should have."
Dest said it was a dream to have contributed to the win with my his goal for Barcelona.
"I tried hard to hold my position because I'm happy in this team," he said, "and I want to keep my place."
Konrad, who debuted for the U.S. national team in the 0-0 tie with Wales, became the seventh American to appear in this season's UEFA Champion League, breaking the record of six set in 2004-05, and the 30th all-time.
Dest and Konrad, who entered the game in the 83rd minute, are the first Americans to be on the field for the same team in a Champions League game since Tim Howard and Jonathan Spector for Manchester United in 2004.