*New Amsterdam FC, Mass United Rush, Lansdowne Boyz, Newtown Pride. Mid-Atlantic:
*Maryland Bobcats, Steel Pulse, Allentown United, Atlantic City FC. Southeast:
*Chattanooga FC, Atletico Atlanta, Savannah Clovers, Soda City FC. Great Lakes:
*Detroit City FC, FC Buffalo, Cleveland SC, Livonia City FC. Midwest:
**Chicago House AC, FC Milwaukee Torrent, Med City FC, Union Dubuque. South Central:
LA Krewe, Alexandria PBFC, Lone Star Republic, Texas Spurs FC. Southwest:
Atletico Olympians, FC Arizona, *Cal United Strikers, *1904 FC. West Coast:
*LA Force, ASC San Diego, Chula Vista FC, Magia Futbol Academy. Pacific:
**Bay Cities FC, California Victory, PDX FC, Space United FC.
*Spring 2021 NISA member. **Future NISA member. Note:
Nine regional champions will be crowned in the competition kicking off on July 9.