Teams from NISA and MLS Next Pro will debut in the U.S. Open Cup this week when play moves into second round. All Division II and Division III teams will be in action. Tuesday, April 5
Charlotte Independence (USLL1) vs. North Carolina Fusion U23 (USLL2) Pittsburgh, Pa.
Pittsburgh Riverhounds SC (USLC) vs. Maryland Bobcats FC (NISA) St. Petersburg, Fla
. Tampa Bay Rowdies (USLC) vs. The Villages SC (USLL2) Hamtramck, Mich
. Detroit City FC (USLC) vs. Michigan Stars FC (NISA) Indianapolis, Ind
. Indy Eleven (USLC) vs. St. Louis CITY 2 (MLSNP) Omaha, Neb.
Union Omaha (USLL1) vs. Des Moines Menace (USLL2) Tulsa, Okla.
FC Tulsa (USLC) vs. Tulsa Athletic (NPSL) San Antonio, Texas
San Antonio FC (USLC) vs. D'Feeters Kicks Soccer Club (Texas) Albuquerque, N.M.
New Mexico United (USLC) vs. Las Vegas Legends FC (NPSL) Clovis, Calif.
Central Valley Fuego FC (USLL1) vs. El Paso Locomotive FC (USLC) Irvine, Calif.
Orange County SC (USLC) vs. Los Angeles Force (NISA) Wednesday, April 6 Montclair, NJ.
Motown FC (NPSL) vs. AC Syracuse Pulse (NISA) Cary, N.C.
North Carolina FC (USLL1) vs. Rio Grande Valley FC Toros (USLC) Mt. Pleasant, S.C.
Charleston Battery (USLC) vs. South Georgia Tormenta FC (USLL1) Miami, Fla.
Miami FC (USLC) vs. Miami United FC (NPSL) Ludlow, Mass.
Western Mass Pioneers (USLL2) vs. Flower City Union (NISA) Louisville, Ky.
Louisville City FC (USLC) vs. Chattanooga Red Wolves SC (USLL1) Madison, Wis.
Forward Madison FC (USLL1) vs. Cleveland SC (NPSL) Colorado Springs, Colo.
Colorado Springs Switchbacks FC (USLC) vs. Northern Colorado Hailstorm FC (USLL1) Tucson, Ariz.
FC Tucson (USLL1) vs. Las Vegas Lights FC (USLC) Chandler, Ariz.
Phoenix Rising FC (USLC) vs. Valley United FC (NISA) San Jose, Calif.
Bay Cities FC (NISA) vs. Monterey Bay F.C. (USLC) San Diego, Calif.
Albion San Diego (NISA) vs. San Diego Loyal SC (USLC) Leesburg, Va.
Nova FC (Va.) vs. Richmond Kickers (USLL1) Thursday, April 7
Hartford Athletic (USLC) vs. Oyster Bay United FC (N.Y.) Rochester, N.Y.
Rochester New York FC (MLSNP) vs. Lansdowne Yonkers FC (N.Y.) Chattanooga, Tenn.
Chattanooga FC (NISA) vs. Memphis 901 FC (USLC) Greenville, S.C.
Greenville Triumph SC (USLL1) vs. Oakland Roots (USLC) Birmingham, Ala.
Birmingham Legion FC (USLC) vs. Southern States SC Stars (NPSL) Sacramento, Calif.
Sacramento Republic FC (USLC) vs. Portland Timbers U23 (USLL2) Irvine, Calif.
California United Strikers FC (NISA) vs. San Fernando Valley FC (Calif.)