The draw for the U.S. Open Cup's round of 32 was held on Thursday.
Teams were drawn from eight groups that included at least one MLS team each that received a third-round bye.
The 32 remaining teams include 24 teams from MLS and eight from the USL Championship.
The round of 32 will take place May 9-10.
Note: Home teams listed first.
Group 1 *Austin FC vs. New Mexico United (USL Championship)
New Mexico United vs. MLS: 2-1.
Houston Dynamo vs. Sporting Kansas City
Group 2 *Nashville SC vs. FC Dallas
Birmingham Legion (USL Championship) vs. Memphis 901 (USL Championship)
Birmingham Legion vs. MLS: 0-0.
Memphis 901 vs. MLS: 1-1.
Group 3 *LA Galaxy vs. Seattle Sounders
Portland Timbers vs. Real Salt Lake
Group 4 Sac Republic FC (USL Championship) vs. Colorado Rapids
Sac Republic FC vs. MLS: 5-6
Monterey Bay F.C. (USL Championship) vs. *LAFC
Monterey Bay F.C. vs. MLS: 1-0
Group 5 Loudoun United (USL Championship) vs. Columbus Crew
Loudoun United vs. MLS: 0-0
FC Cincinnati vs. *New York City FC
Group 6 *NY Red Bulls vs. D.C. United
New England Revolution vs. Pittsburgh Riverhounds (USL Championship)
Pittsburgh Riverhounds vs. MLS: 1-5.
Group 7 Charlotte FC vs. *Orlando City
Inter Miami vs. Charleston Battery (USL Championship)
Charleston Battery vs. MLS: 9-15
Group 8 Chicago Fire vs. St. Louis City
Minnesota United vs. *Philadelphia Union
*Third-round bye.